• Current location:homepage  Facilities  Nano-Bio Platform
  • Real-Time PCR Detection System

    Author: Release time:2017-07-13 Views:2213



    Instrument name

    Real-Time PCR Detection System

    Manufacturer

    BIO-RAD

    Type number

    CFX Connect

    Specifications

    1.Channel number: 3; 2 target genes and 1 FRET   channels can    be detected simultaneously.

    2.Excitation light source: high intensity LED lamp; Detector: PTD.

    3.Excitation / emission wavelength range: 450-580   nm.

    4.Sample number: 96 well; Sample carrier: 96 well plate, pipe, span></p>

      &nbsp;PCR pipe.

    5.Sample Volume: 1-50µl.

    6.Suitable for a variety of fluorescence methods: Taqman, span></p>

    Molecular Beacon, FRET Probe, SYBR Green I.

    7.Temperature Gradient: With 8 dynamic temperature gradient span></p>

    &nbsp;PCR function.

    8.Maximum Block Ramp Rate: 5 °C/s.

    9.Temperature Range: 0-100 °C; Temperature Accuracy: ±0.2 °C;

    Temperature Uniformity: ±0.4 °C.

    10.Dynamic Range: no less than 10 orders of magnitude.

    11.Sensitivity: to detect single copy gene in human genome.

    Function and applications

    The fluorescent quantitative PCR is widely used, which can be

    used in :

    1. Absolute quantification of nucleic acids (DNA or RNA);

    2. Analysis of gene expression level;

    3. detection of gene mutation;

    4. GMO detection and product specificity analysis;

    5. Genotyping: for example, SNP detection, methylation detection and other research areas.

    Accessories

    None

    Contacts:Mudan Gan

    Contact number: 65880223

    E-mail: gmd@suda.edu.cn



    Editor: Mudan Gan