报告人： Prof. Anne Andrews

University of California, Los Angeles

题目：Nanoengineered Substrates for Nucleic Acid Biorecognition

时间：2015年8月29日（星期六）10:15am

地点：Conference Room B, BLDG 909-1F

摘要:

Recently we reported on the development of chemical lift-off lithography for patterning self-assembled monolayers on Au substrates over scales ranging from tens of nanometers to centimeters. Here, we took advantage of the residual alkanethiols remaining in the patterned regions after PDMS stamp lift-off to serve as environments capable of supporting insertion of single-stranded thiolated DNA. We found that the composition of alkanethiol matrices influences the degree of DNA insertion. Patterned DNA was biospecifically recognized by complementary fluorescently labeled DNA. Moreover, hybridization efficiency was greater for substrates prepared via lift-off lithography compared to self-assembled DNA alone or with alkanethiol backfilling. We have also patterned self-assembled monolayer substrates using a microfluidics approach wherein individual channels are used to functionalize substrates with different small molecule probes. These substrates recognized DNA binding partners (aptamers) and readily distinguished single base-pair sequence alterations. We are currently using neurotransmitter-molecule functionalized self-assembled monolayer substrates to screen nucleic acid libraries for new higher affinity small-molecule aptamers for in vivo biosensing applications.  

简介:

Anne Andrews, Ph.D., is Shirley M. Hatos Endowed Chair in Clinical Neuropharmacology, and Professor of Psychiatry & Biobehavioral Sciences and Chemistry & Biochemistry at the University of California, Los Angeles. At UCLA, Andrews leads efforts in basic and translational research on anxiety and depression, and at the nexus of nanoscience and neuroscience. Andrews’ interdisciplinary research team focuses on understanding how the serotonin system and particularly, the serotonin transporter, modulate neurotransmission to influence complex behaviors including anxiety, mood, stress responsiveness, and learning and memory.

Dr. Andrews earned her B.S. in chemistry from the Pennsylvania State University and received her Ph.D. in chemistry as a U.S. Department of Education Fellow working at the National Institute of Mental Health. There, she was later a postdoctoral fellow and senior staff fellow. Andrews has been the recipient of an NIH Fellows Award for Research Excellence, an Eli Lily Outstanding Young Analytical Chemist Award, an American Parkinson’s Disease Association Research Award, and a Brain and Behavior Research Foundation Independent Investigator Award. She is a member of the American College of Neuropsychopharmacology, a fellow of the Collegium Internationale Neuropsychopharmacologicum, International Society for Serotonin Research vice president, an advisory board member for the International Society for Monitoring Molecules in Neuroscience, and serves as Associate Editor for ACS Chemical Neuroscience.