题目：

A real-time documentation and mechanistic investigation of quantum dots-induced autophagy in live Caenorhabditis elegans

作者：

Yanfeng Zhou ^{a, b, 1}, Qin Wang ^{a, b, 1}, Bin Song ^a, Sicong Wu ^a, Yuanyuan Su ^a, Huimin Zhang ^{b, **}, Yao He ^{a, *}

单位：

^a Institute of Functional Nano & Soft Materials (FUNSOM) and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China.

^b Institutes of Biology and Medical Sciences (IBMS), Soochow University, Suzhou 215123, China.

摘要：

Autophagy is known as highly important intracellular process for the degradation of endogenous or foreign contents from the cytoplasm. While nanomaterials-induced autophagy has been recently reported, real-time information about autophagosomes formation and in situ autophagy behaviors induction by nanomaterials in live organisms nevertheless remain poorly understood. Herein, by using C. elegans as a platform and fluorescent semiconductor quantum dots (QDs) as a representative kind of nanomaterials, the phenomenon of QDs-induced autophagy in live organisms is systematically investigated. Specifically, our results demonstrate that the internalized QDs can significantly up-regulate the autophagy level in intestinal cells of C. elegans. And our further studies reveal that this QD-related autophagy in C. elegans is neither a response to released heavy metal ions by the QDs, nor an attempt to engulf exogenous QD materials, but a standard procedure of the organism to clear and recycle damaged cellular organelles. Of particular significance, for the first time, we further present real-time in situ tracking of autophagosomes formation in live organisms, providing detailed temporal-spatial information of this process. Collectively, this study helps us understanding the relationship between nanomaterials and autophagy in vivo, and provides invaluable information for safety evaluation and further practical bio-application of nanomaterials.

影响因子：

8.557

分区情况：

1区

链接：

http://www.sciencedirect.com/science/article/pii/S0142961215007188