题目：

Simultaneous Isolation and Detection of Circulating Tumor Cells with a Microfluidic Silicon-Nanowire-Array Integrated with Magnetic upconversion nanoprobes

作者：

Chao Wanga,^a,1 Min Yea,^a,1 Liang Cheng^a, Rui Li^b, Wenwen Zhu^a, Zhen Shi^b, Chunhai Fan^c, Jinkang Heb,* Jian Liua,* Zhuang Liua,*

单位：

^aInstitute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China

^bDepartment of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China

^cLaboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academic of Sciences, Shanghai 201800, China

摘要：

The development of sensitive and convenient methods for detection, enrichment, and analysis of circulating tumor cells (CTCs), which serve as an importance diagnostic indicator for metastatic progression of cancer, has received tremendous attention in recent years. In this work, a new approach characteristic of simultaneous CTC capture and detection is developed by integrating a microfluidic silicon nanowire (SiNW) array with multifunctional magnetic upconversion nanoparticles (MUNPs). The MUNPs were conjugated with anti-EpCAM antibody, thus capable to specifically recognize tumor cells in the blood samples and pull them down under an external magnetic field. The capture efficiency of CTCs was further improved by the integration with a microfluidic SiNW array. Due to the autofluorescence free nature in upconversion luminescence (UCL) imaging, our approach allows for highly sensitive detection of small numbers of tumor cells, which afterward could be collected for further analysis and re-culturing. We have further demonstrated that this approach can be applied to detect CTCs in clinical blood samples from lung cancer patients, and obtained consistent results by analyzing the UCL signals and the clinical outcomes of lung cancer metastasis. Therefore our approach represents a promising platform in CTC capture and detection with potential clinical utilization in cancer diagnosis and prognosis.

影响因子：

8.312

分区情况：

1区

链接：

http://www.sciencedirect.com/science/article/pii/S0142961215002744