题目：

Enhanced Proliferation and Osteogenic Differentiation of Human Mesenchymal Stem Cells on Biomineralized Three-Dimensional Graphene Foams

作者：

Ting Zhang^a, Ning Li^c, Kunyang Li^a, Ruifang Gao^a, Wei Gu^a, Chengcheng Wu^a, Ruigong Su^d, Liwei Liu^d, Qi Zhang^a,b,*, Jian Liu^a,**

单位：

^aJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China

^bSchool of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China

^cCenter for Cancer and Immunology Research, Children’s National Medical Center, Washington DC 20010, USA

^dSuzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Jiangsu 215123, China

摘要：

Human mesenchymal stem cells (hMSCs) hold great promise for bone regeneration, yet the direction of MSC proliferation and induction of MSC differentiation remain challenging. Very recently, grapheme provides a novel substrate for cell culture. Here, we report the utilization of mineralized three dimensional graphene (3DG) scaffolds for hMSC growth. The nano-structured hydroxyapatite (HA) particles decorated 3D graphene (HA-3DG) scaffolds were developed by mineralization in 10 times concentrated simulated body fluid (10SBF) containing 10 mM of HCO₃^-. The HA-3DG scaffolds showed higher roughness and cell proliferation compared with the 2D graphene films. More importantly, the mineralized 3DG scaffolds exhibited faster osteogenic commitment and stronger osteogenic differentiation (13.7, 10.9 and 1.89 fold at 7d, 10d and 14d respectively from the western blot analysis). These findings demonstrated the potential of mineralized 3DG scaffold as a promising platform for hMSC culture and bone regeneration.

影响因子：

6.196

分区情况：

1区

链接：

http://www.sciencedirect.com/science/article/pii/S0008622316302883