题目：

Serum-Resistant, Reactive Oxygen Species (ROS)-Potentiated Gene Delivery in Cancer Cells Mediated by Fluorinated, Diselenide- Crosslinked Polyplexes

作者：

Qiurong Deng,‡^a Xudong Li,‡^a Lipeng Zhu,^a Hua He,^a Donglai Chen,^bYongbing Chen^c and Lichen Yin *^a

单位：

^aJiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM), Soochow University, Suzhou 215123, P.R. China.

^bDepartment of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, P.R. China.

^cDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou 215004, P.R. China.

摘要：

The transfection performance of polycations is often hampered by various systemic barriers that pose conflicting requirements for material design. Herein, we developed fluorinated, ROS-cleavable polyethylenimine (PEI) for effective and serum-resistant gene delivery to cancer cells, by harmonizing the inconsistency between DNA condensation and release, and the inconsistency between cellular internalization and serum stability. Low-molecular weight (MW) PEI was cross-linked with a diselenide-containing linker and further modified with fluorocarbon chains. The obtained high-MW DSe-PEI-F has potent DNA condensation as well as intracellular DNA delivery capabilities, while in the cytoplasm of cancer cells, it can rapidly degrade into low-MW segments upon ROS treatment to promote DNA release and reduce the material toxicity. As such, DSe-PEI-F showed high transfection efficiencies in cancer cells in the presence of serum, outperforming the commercial reagent PEI 25k by several orders of magnitude. This study thus provides an effective approach to overcome various barriers against non-viral gene delivery, which contributes to the development of a new class of gene vectors with high efficiency and

low toxicity.

影响因子：

4.21

分区情况：

二区

链接：

http://pubs.rsc.org/-/content/articlehtml/2017/bm/c7bm00334j