题目：

DNA-Edited Ligand Positioning on Red Blood Cells to Enable Optimized T Cell Activation for Adoptive Immunotherapy

作者：

Lele Sun,¹ Fengyun Shen,¹ Jun Xu,¹ Xiao Han,¹ Chunhai Fan² and Zhuang Liu^1,*

单位：

¹Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, Suzhou, 215123, Jiangsu (China)

²School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, 201240 (China)

摘要：

Artificial antigen presenting cells (aAPCs) with surface-anchored T cell activating ligands hold great potential in adoptive immunotherapy. However, it remains challenging to precisely control the ligand positioning on those platforms using conventional bioconjugation chemistry. Utilizing DNA-assisted bottom-up self-assembly, we were able to precisely control both lateral and vertical distributions of T cell activation ligands on red blood cells (RBCs). The clustered lateral positioning of the peptide-major histocompatibility complex (pMHC) on RBCs with a short vertical distance to the cell membrane is favorable for more effective T cell activation, likely owing to their better mimicry of natural APCs. Such optimized RBC-based artificial APCs can stimulate T cell proliferation in vivo and effectively inhibit tumor growth with adoptive immunotherapy. DNA technology is thus a unique tool to precisely engineer the cell membrane interface and tune cell–cell interactions, which is promising for applications such as immunotherapy.

影响因子：

12.959

分区情况：

一区

链接：

https://onlinelibrary.wiley.com/doi/full/10.1002/anie.202003367

责任编辑：向丹婷