| 作者： | Hua He,† Nan Zheng,‡ Ziyuan Song,‡ Kyung Hoon Kim,‡ Catherine Yao,‡ Rujing Zhang,‡ Chenglin Zhang,§ Yuhui Huang,§ Fatih M. Uckun,⊥ Jianjun Cheng,‡ Yanfeng Zhang,∥ and Lichen Yin*,† |
| 单位： | †Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 199 Ren’ai Road, Suzhou 215123, China ‡Department of Materials Science and Engineering, University of Illinois at Urbana�Champaign, 1304 West Green Street, Urbana, Illinois 61801, United States §The Cyrus Tang Hematology Center, The Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215123, China ⊥Division of Hematology-Oncology, Systems Immunobiology Laboratory, Children’s Center for Cancer and Blood Diseases, Children’s Hospital, Los Angeles, California 90027, United States ∥Department of Applied Chemistry, School of Science, Xi’an Jiaotong University, Xi’an 710049, China |
| 摘要： | Treatment of inflammatory diseases represents one of the biggest clinical challenges. RNA interference (RNAi) against TNF-α provides a promising modality toward anti-inflammation therapy, but its therapeutic potential is greatly hampered by the by the lack of efficient siRNA delivery vehicles in vivo. Herein, we report a hybrid nanoparticulate (HNP) system based on a cationic helical polypeptide PPABLG for the efficient delivery of TNF-α siRNA. The helical structure of PPABLG features pore formation on cellular and endosomal membranes to facilitate the direct translocation as well as endosomal escape of TNF-α siRNA in macrophages, representing a unique superiority to a majority of the existing polycation-based gene vectors that experience severe endosomal entrapment and lysosomal degradation. As such, HNPs containing TNF-α siRNA afforded effective systemic TNF-α knockdown following systemic administration at a low dose of 50 µg of siRNA/kg and thus demonstrated a potent anti-inflammatory effect to rescue animals from LPS/D-GalN-induced hepatic sepsis. This study therefore verifies that the bioactive secondary structure of polypeptides significantly dominates the in vivo siRNA delivery efficiency, and the unique properties of PPABLG HNPs render remarkable potentials for anti-inflammation therapies. |
