题目：

Ultrasensitive Signal-On Detection of Nucleic Acids with Surface- Enhanced Raman Scattering and Exonuclease III-Assisted Probe Amplification

作者：

Yingying Li,^§,† Qingcheng Zhao,^§,† Yandong Wang,^† Tiantian Man,^‡ Lu Zhou,^*,† Xu Fang,^† Hao Pei,^‡ Lifeng Chi,^† and Jian Liu^*,†

单位：

^† Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu 215123, China.

^‡ School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China.

摘要：

The methodological development of nucleic acids detection is a rapidly growing research field. Here, we report a powerful method to detect nucleic acids by an integration of surface-enhanced Raman scattering and exonuclease III-assisted probe amplification. With a unique signal-on strategy, we have demonstrated that the target DNA of MnSOD gene in concentrations as low as 1 aM can reproducibly be detected, which offers a detection limit several orders of magnitude better than the previous reports in the literature. The new biosensor exhibits an excellent specificity in differentiating DNA sequences with a single-base mismatch. As a robust, flexible, and ultrasensitive approach, it promises important applications in clinical diagnostics and DNA identification where only a very limited amount of the biological sample is available.

影响因子：

5.886

分区情况：

二区

链接：

http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03267

责任编辑：向丹婷 联系方式：dtxiang@suda.edu.cn