题目：

Simultaneous Enzymatic Activity Modulation and Rapid Determin- ation of Enzyme Kinetics by Highly Crystalline Graphite Dots

作者：

Rui Shi^{a, §}, Hao Li^{a, §}, Enhui Wu^a, Lipeng Xiong^b, Rui Lv^a, Ruochen Guo^a, Yang Liu^a, GuoqiangXu^b, Zhenhui Kang^a*, Jian Liu^a*

单位：

^aJiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu 215123, China.

^bCollege of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215123, China.

摘要：

The research field in enzyme-based biotechnology urgently requests discovery of new materials and methods with highperformance. Here we report that highly crystalline graphite dots (GDs) can modulate enzyme activities, and simultaneously allow for real-time measurements on enzyme kinetics in a combination with mass spectrometry (MS). A well-defined modulation of lipolytic activities from inhibition to enhancement can be realized by selectively coupling lipase enzymes with GDs containing specific functional groups on the surface. As a unique feature of our approach, GDs in the enzyme reaction can simultaneously serve as a versatile matrix for rapid and sensitive detection of the residual enzyme substrate, the intermediate or final product of lipolytic digestion using MS technology. Therefore, enzyme kinetic data can be collected in a real-time, high-throughput format. This work provides a new platform for enzymological research in hybrid bio-catalytic processes with the advanced nanotechnology.

影响因子：

7.760

分区情况：

一区

链接：

http://pubs.rsc.org/en/content/articlelanding/2017/nr/c7nr02285a#!divAbstract

责任编辑：向丹婷 联系方式：dtxiang@suda.edu.cn