题目：

Millisecond-Range Time-Resolved Bioimaging Enabled through Ultralong Aqueous Phosphorescence Probes

作者：

Mingyue Cui ¹, Peiling Dai ², Jiali Ding ¹, Manjing Li ¹, Rong Sun ¹, Xin Jiang ¹, Menglin Wu ¹, Xueke Pang ¹, Mingzhu Liu ¹, Qiang Zhao ², Bin Song ^1,*,  Yao He ^1,*

单位：

¹Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow   University, Jiangsu, Suzhou 215123 (China)

²State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for  Biosensors, Institute of Advanced Materials (IAM) & Institute of Flexible Electronics (Future Technology), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023 (China)

摘要：

Probes featuring room-temperature phosphorescence (RTP) are promising tools for time-resolved imaging. Itis worth noting that the time scale of time-resolved bioimaginggenerally ranges around the microsecond level, because of the short-lived emission. Herein, the first example of millisecondrange time-resolved bioimaging is illustrated, which is enabled through a kind of ultralong aqueous  phosphorescence probes (i.e., cyclo-(Arg-Gly-AspD-Tyr-Cys)-conjugated zinc-doped silica nanospheres), with a RTP emission lasting for ~5 s and a lifetime as long as 743.7 ms. We demonstrate that live cells and deep tumor tissue in mice can be specifically targeted through immune-phosphorescence imaging, with a high signal-to-background ratio (SBR) value of ~69 for in vitro imaging, and ~627 for in vivo imaging, respectively. We further show that, compared to that of fluorescence imaging, the SBR enhancement of millisecondrange time-resolved in vivo bioimaging is up to 105 times.

影响因子：

15.336

分区情况：

一区

链接：

https://doi.org/10.1002/ange.202200172